Journal: PLoS ONE

Article Title: Functional Cooperativity between ABCG4 and ABCG1 Isoforms

doi: 10.1371/journal.pone.0156516

Figure Lengend Snippet: (A) HEK293 cells were transiently transfected with ABCG4 (G4), ABCG1 (G1), ABCG2 (G2), or with catalytic site mutant variants of ABCG4 and ABCG1 (G4KM, G1KM). The cell growth of these cultures was monitored for 72 hours. Cells expressing the wild type ABCG4 or ABCG1 protein showed inhibited growth capacity as compared to cells expressing the inactive mutants or the wild type ABCG2 (G2). (B) The expression level of ABCG proteins was also assessed by Western analysis. The total expression of the transgene rapidly declined in the cultures transfected with the wild type ABCG4 or ABCG1, whereas stable expression levels were observed in cells expressing the inactive mutants or the wild type ABCG2. Numbers at the top indicate the elapsed time in hours. (C) Following a three week long selection with G418, no ABCG4 expression was detected in cultures transfected with wild type protein (G4), whereas expression of the inactive mutant (G4KM) persisted. For loading control Na + K + ATPase was used.

Article Snippet: For loading control, a mouse anti-Na + K + ATPase antibody (Biomol, 1:1600) was used, with the exception when the entire cells lysates were subjected to Western blot analysis.

Techniques: Transfection, Mutagenesis, Expressing, Western Blot, Selection, Control

Journal: Cells

Article Title: Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum

doi: 10.3390/cells10113038

Figure Lengend Snippet: Antibodies used for Western Blot.

Article Snippet: Na + /K + -ATPase , Mouse-anti-Na + /K + -ATPase , Enzo Life Sciences AG 804-082 , 1:100 , Goat-anti-mouse HRP , Invitrogen/A16072 , 1:5000.

Techniques: Western Blot

Journal: Cells

Article Title: Proliferation and Differentiation of Intestinal Caco-2 Cells Are Maintained in Culture with Human Platelet Lysate Instead of Fetal Calf Serum

doi: 10.3390/cells10113038

Figure Lengend Snippet: Protein expression of SGLT1 ( a ), GLUT2 ( b ), v ( c ) and Na + /K + -ATPase ( d ) was assessed by Western blot analysis in order to evaluate differentiation of the cells after growth with the different supplements. Boxes represent medians ±10th, 25th, 75th and 90th percentile; N = 3, one-way RM ANOVA + Holm–Sidak test, p < 0.05. Different letters (A, B) indicate significant differences between groups. Representative blots are shown below. GLUT: glucose transporter, SGLT1: Na+-coupled glucose transporter 1.

Article Snippet: Na + /K + -ATPase , Mouse-anti-Na + /K + -ATPase , Enzo Life Sciences AG 804-082 , 1:100 , Goat-anti-mouse HRP , Invitrogen/A16072 , 1:5000.

Techniques: Expressing, Western Blot